Review



atm ps1981  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc atm ps1981
    Atm Ps1981, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atm+ps1981/pm41634336-249-37-39
    Average 86 stars, based on 1 article reviews
    atm ps1981 - by Bioz Stars, 2026-11
    86/100 stars

    Images

    Related Articles

    other:

    Article Title: DGCR8 regulates multiple processes of transcription coupled nucleotide excision repair.
    Article Snippet: The following commercial antibodies were also used: DGCR8 (10996-1-AP, Proteintech; sc-377249, Santa Cruz), RNA polymerase II (CDT-pS5, sc-55492[F-12], Santa Cruz), CSB (sc-25370, Santa Cruz), CSA (sc376981, Santa Cruz), USP7 (300-033, Bethyl), UVSSA (GT816, GeneTex), CHK1-pS345 (2341L, CST), ATM-pS1981 (4526S, CST), SPT16 (sc-165987, Santa Cruz), SMARCA5 (sc365727, Santa Cruz), CPD (CAC-NM-DND-001, Cosmo Bio), 6-4PP (CAC-NM-DND-002, Cosmo Bio), and GFP (50430-2-AP, Proteintech; AE012, Abclonal).

    Article Title: An ATM-PPM1D Circuit Controls the Processing and Restart of DNA Replication Forks
    Article Snippet: Primary antibodies include: β-actin (Proteintech, #66009-1-lg), β-tubulin (Proteintech, #66240-1-lg), Histone H4 (Bethyl Laboratories, #A300-647A), KAP1-pS824 (Bethyl Laboratories, #304-146A), Gamma-H2AX (Bethyl Laboratories, #A300-081A), RPA32 (Bethyl Laboratories, #A300-244A), PCNA (Santa Cruz Biotechnology, #sc-56), CHK1-pS345 (133D3, Cell Signaling Technology, #2348), PPM1D/WIP1 (Santa Cruz Biotechnology, #sc-376257), ATM-pS1981 (Cell Signaling Technology, #4526), 53BP1 (Cell Signaling Technology #4937), RAD51 (Millipore Sigma, #PC130), Rat anti-BrdU (BU1/75 (ICR1) (Abcam, #6326), Mouse anti-BrdU (B44) (BD Biosciences, #347580).

    Article Title: Differential regulation of Retinoic Acid Metabolism in Fanconi Anemia
    Article Snippet: The following antibodies were used: rabbit polyclonal antisera against AKR1C3 (11194-1-AP; Proteintech), ALDH2 (18818S; Cell Signaling), ATR pS428 (2853S; Cell Signaling), CRABP2 (10225-1-AP; Proteintech), FANCD2 (NB100-182; Novus Biologicals), MRE11 (PC388; Calbiochem), PARP (9542; Cell Signaling), and RDH10 (14644-1-AP; Proteintech), or rabbit monoclonal antisera against ALDH1A1 (54135; Cell Signaling), ATM pS1981 (5883; Cell Signaling), ATR (13934; Cell Signaling), Caspase-3 (14220; Cell Signaling), Caspase-7 (12827; Cell Signaling), CHK1 pS345 (2348T; Cell Signaling), CHK2 pT68 (2197; Cell Signaling), Cleaved Caspase-3 (D175) (9664; Cell Signaling), Cleaved Caspase-7 (D198) (8438; Cell Signaling), Cleaved PARP (D214) (5625; Cell Signaling), and H2AX pS139 ( γ H2AX) (9718; Cell Signaling), or mouse monoclonal antisera against alpha-tubulin (MS-581-PO; Neomarkers), ATM (11G12) (92356; Cell Signaling), CHK1 (2G1D5) (2360; Cell Signaling), p53 (DO-1) (F0909; Santa Cruz Biotechnology), p53 pS15 (9286; Cell Signaling), and RPA2 (NA18; Calbiochem).

    Article Title: DGCR8 regulates multiple processes of transcription coupled nucleotide excision repair
    Article Snippet: The following commercial antibodies were also used: DGCR8 (10996-1-AP, Proteintech; sc-377249, Santa Cruz), RNA polymerase II (CDT-pS5, sc-55492[F-12], Santa Cruz), CSB (sc-25370, Santa Cruz), CSA (sc-376981, Santa Cruz), USP7 (300 − 033, Bethyl), UVSSA (GT816, GeneTex), CHK1-pS345 (2341 L, CST), ATM-pS1981 (4526 S, CST), SPT16 (sc-165987, Santa Cruz), SMARCA5 (sc-365727, Santa Cruz), CPD (CAC-NM-DND-001, Cosmo Bio), 6-4PP (CAC-NM-DND-002, Cosmo Bio), and GFP (50430-2-AP, Proteintech; AE012, Abclonal).

    Ubiquitin Proteomics:

    Article Title: Mechanism and function of DNA replication‐independent DNA‐protein crosslink repair via the SUMO‐RNF4 pathway
    Article Snippet: .. Antibodies to human proteins used in this study included: actin (clone C4, MAB1501, Merck (1:20,000 dilution), RRID:AB_2223041); ATM (ab78, Abcam (1:1,000), RRID:AB_306089); ATM‐pS1981 (4526, Cell Signaling Technology (1:1,000), RRID:AB_2062663); CDC27 (sc‐9972, Santa Cruz, RRID:AB_627228); CDK1‐pY15 (ab47594, Abcam (1:1,000), RRID:AB_869073); CHK1 (sc‐8408, Santa Cruz (1:500), RRID:AB_627257); CHK1‐pS317 (2344S, (Cell Signaling Technology (1:1,000), RRID:AB_331488); cyclin A (sc‐751, Santa Cruz (1:1,000), RRID:AB_631329); cyclin B (610220, BD Bioscience (1:1,000), RRID:AB_397617); GFP (11814460001 (clones 7.1 and 13.1), Roche (1:1,000), RRID:AB_390913; ab6556, Abcam (1:5,000), RRID:AB_305564); histone H2AX (2595S, Cell Signaling Technology (1:1,000), RRID:AB_10694556); histone γ‐H2AX (05‐636, Millipore (1:1,000), RRID:AB_309864); histone H3 (ab1791, Abcam (1:50,000), RRID:AB_302613); histone H3‐pS10 (06‐570, Millipore (1:1,000), RRID:AB_310177); KAP1 (A300‐274A, Bethyl Laboratories (1:1,000), RRID:AB_185559); KAP1‐pS824 (A300‐767A, Bethyl (1:1,000), RRID:AB_669740); NF‐κB p65 (sc‐372, Santa Cruz (1:2,000); RRID:AB_632037); RNF4 (Maure et al , ) (1:6000)); PIAS4 (sc‐166706, Santa Cruz (1:1,000); RRID: AB_2164374); SUMO2/3 (ab3742, Abcam (1:1,000), RRID:AB_304041; ab81371 (8A2), Abcam (1:200), RRID:AB_1658424); ubiquitin (sc‐8017 (P4D1), Santa Cruz (1:1,000), RRID:AB_628423); ubiquitin(K48) (05‐1307, Millipore (1:1,000), RRID:AB_1587578). ..

    Western Blot:

    Article Title: KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells
    Article Snippet: .. The antibodies used for Western blot analysis were ATM (Abcam, Cambridge, UK), ATM-pS1981 (Cell Signaling Technology, MA, USA), KAP1 (Novus Biologicals, CO, USA), KAP1-pS824 (Novus Biologicals), CHK2 (Millipore, MA, USA), CHK2-pT68 (Novus Biologicals), γ-H2AX (Cell Signaling Technology), p53 (Santa Cruz Biotechnology, TX, USA), p53-pS15 (Cell Signaling Technology), MDM2 (Santa Cruz Biotechnology), GAPDH (Genetex, CA, USA), and histone H3 (Abcam). .. Quantified data were analyzed statistically using Prism Software (GraphPad, CA, USA).

    Cell-Signaling:

    Article Title: Wild-type IDH2 protects nuclear DNA from oxidative damage and is a potential therapeutic target in colorectal cancer.
    Article Snippet: Although the role of isocitrate dehydrogenase (IDH) mutation in promoting cancer development has been well-characterized, the impact of wild-type IDH on cancer cells remains unclear.. Here we show that the wild-type isocitrate dehydrogenase 2 (IDH2) is highly expressed in colorectal cancer (CRC) cells, and plays an unexpected role in protecting the cancer cells from oxidative damage.. Genetic abrogation of IDH2 in CRC cells leads to reactive oxygen species (ROS)-mediated DNA damage and an accumulation of 8-oxoguanine with DNA strand breaks, which activates DNA damage response (DDR) with elevated γH2AX and phosphorylation of ataxia telangiectasia-mutated (ATM) protein, leading to a partial cell cycle arrest and eventually cell senescence.

    Article Title: Pyruvate Facilitates FACT‐Mediated γ H2AX Loading to Chromatin and Promotes the Radiation Resistance of Glioblastoma
    Article Snippet: Antibodies : Mouse monoclonal antibodies against Tubulin (1:5000, T5201), Flag (1:5000, F3165) and anti‐Flag M2 affinity gel (A2220) were purchased from Sigma (St. Louis, MO, USA). .. Rabbit monoclonal antibodies against PKM2 (1:3000, 4053S), HA (1:3000, 3724S), β ‐actin (1:3000, 3700S), LaminB1 (1:3000, 12586s), H2AX pS139 (1:3000, 9718S), ATM pS1981 (1:1000, 13050S), ATM (1:1000, 2873), AKT pT308 (1:1000, 4056S), and rabbit polyclonal antibody against EGFR pY1068 (1:1000, 2234S) were obtained from Cell Signaling Technology (Danvers, MA, USA). .. Rabbit polyclonal antibody against histone H3 (1:2000, ab1791) were purchased from Abcam (Cambridge, USA).

    Bioprocessing:

    Article Title: Pyruvate Facilitates FACT‐Mediated γ H2AX Loading to Chromatin and Promotes the Radiation Resistance of Glioblastoma
    Article Snippet: Antibodies : Mouse monoclonal antibodies against Tubulin (1:5000, T5201), Flag (1:5000, F3165) and anti‐Flag M2 affinity gel (A2220) were purchased from Sigma (St. Louis, MO, USA). .. Rabbit monoclonal antibodies against PKM2 (1:3000, 4053S), HA (1:3000, 3724S), β ‐actin (1:3000, 3700S), LaminB1 (1:3000, 12586s), H2AX pS139 (1:3000, 9718S), ATM pS1981 (1:1000, 13050S), ATM (1:1000, 2873), AKT pT308 (1:1000, 4056S), and rabbit polyclonal antibody against EGFR pY1068 (1:1000, 2234S) were obtained from Cell Signaling Technology (Danvers, MA, USA). .. Rabbit polyclonal antibody against histone H3 (1:2000, ab1791) were purchased from Abcam (Cambridge, USA).

    Hemagglutination Assay:

    Article Title: Pyruvate Facilitates FACT‐Mediated γ H2AX Loading to Chromatin and Promotes the Radiation Resistance of Glioblastoma
    Article Snippet: Antibodies : Mouse monoclonal antibodies against Tubulin (1:5000, T5201), Flag (1:5000, F3165) and anti‐Flag M2 affinity gel (A2220) were purchased from Sigma (St. Louis, MO, USA). .. Rabbit monoclonal antibodies against PKM2 (1:3000, 4053S), HA (1:3000, 3724S), β ‐actin (1:3000, 3700S), LaminB1 (1:3000, 12586s), H2AX pS139 (1:3000, 9718S), ATM pS1981 (1:1000, 13050S), ATM (1:1000, 2873), AKT pT308 (1:1000, 4056S), and rabbit polyclonal antibody against EGFR pY1068 (1:1000, 2234S) were obtained from Cell Signaling Technology (Danvers, MA, USA). .. Rabbit polyclonal antibody against histone H3 (1:2000, ab1791) were purchased from Abcam (Cambridge, USA).



    Similar Products

    88
    Rockland Immunochemicals anti atm protein kinase ps1981 antibody
    Anti Atm Protein Kinase Ps1981 Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atm+ps1981/Anti-ATM+Protein+Kinase/pm41898390-167-0-5
    Average 88 stars, based on 1 article reviews
    anti atm protein kinase ps1981 antibody - by Bioz Stars, 2026-11
    88/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc atm ps1981
    Atm Ps1981, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atm+ps1981/pm41634336-249-37-39
    Average 86 stars, based on 1 article reviews
    atm ps1981 - by Bioz Stars, 2026-11
    86/100 stars
      Buy from Supplier

    93
    Rockland Immunochemicals anti atm ps1981
    Anti Atm Ps1981, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atm+ps1981/Anti-phospho-ATM+Protein+Kinase+pS1981/pmc12727681-83-0-12
    Average 93 stars, based on 1 article reviews
    anti atm ps1981 - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Rockland Immunochemicals chip
    Chip, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atm+ps1981/Anti-phospho-ATM+Protein+Kinase+pS1981/pmc12727681-83-10-12
    Average 93 stars, based on 1 article reviews
    chip - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Rockland Immunochemicals mouse anti atm ps1981
    Mouse Anti Atm Ps1981, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atm+ps1981/ATM+phospho+S1981+Biotin+Conjugated+Antibody/pmc12727681-146-0-4
    Average 93 stars, based on 1 article reviews
    mouse anti atm ps1981 - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Rockland Immunochemicals mouse anti atm ps1981 rockland
    Mouse Anti Atm Ps1981 Rockland, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atm+ps1981/ATM+phospho+S1981+Biotin+Conjugated+Antibody/pm41348540-832-22-25
    Average 93 stars, based on 1 article reviews
    mouse anti atm ps1981 rockland - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Rockland Immunochemicals s1981
    ( A ) Quantification of the percentage of phosphorylated Chk2 (pChk2) (T68), and pATM <t>(S1981)-positive</t> cells in HCT116 colorectal cancer (CRC) cells incubated with FOLFIRI (5-FU: 6 µM and SN-38: 50 nM) and increasing concentrations of AZD2858 (from 250 to 1000 nM) for 2 hr and ( B ) of pChk1 and γH2AX-positive cells after incubation for 20 hr. Data were obtained by immunofluorescence analysis using the ‘Expression analysis’ application of the Celigo Imaging Cytometer (Nexcelom). The combination of FOLFIRI and AZD2858 is shaded in pink. The experiment was replicated three times, and each point represents a biological replicate. The statistical significance was determined by linear modeling analysis interrogating the effect of each drug separately and of their interaction (p-values given in the inset).
    S1981, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atm+ps1981/Anti-phospho-ATM+Protein+Kinase+pS1981/pmc12539802-204-40-41
    Average 93 stars, based on 1 article reviews
    s1981 - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Quantification of the percentage of phosphorylated Chk2 (pChk2) (T68), and pATM (S1981)-positive cells in HCT116 colorectal cancer (CRC) cells incubated with FOLFIRI (5-FU: 6 µM and SN-38: 50 nM) and increasing concentrations of AZD2858 (from 250 to 1000 nM) for 2 hr and ( B ) of pChk1 and γH2AX-positive cells after incubation for 20 hr. Data were obtained by immunofluorescence analysis using the ‘Expression analysis’ application of the Celigo Imaging Cytometer (Nexcelom). The combination of FOLFIRI and AZD2858 is shaded in pink. The experiment was replicated three times, and each point represents a biological replicate. The statistical significance was determined by linear modeling analysis interrogating the effect of each drug separately and of their interaction (p-values given in the inset).

    Journal: eLife

    Article Title: TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer

    doi: 10.7554/eLife.106196

    Figure Lengend Snippet: ( A ) Quantification of the percentage of phosphorylated Chk2 (pChk2) (T68), and pATM (S1981)-positive cells in HCT116 colorectal cancer (CRC) cells incubated with FOLFIRI (5-FU: 6 µM and SN-38: 50 nM) and increasing concentrations of AZD2858 (from 250 to 1000 nM) for 2 hr and ( B ) of pChk1 and γH2AX-positive cells after incubation for 20 hr. Data were obtained by immunofluorescence analysis using the ‘Expression analysis’ application of the Celigo Imaging Cytometer (Nexcelom). The combination of FOLFIRI and AZD2858 is shaded in pink. The experiment was replicated three times, and each point represents a biological replicate. The statistical significance was determined by linear modeling analysis interrogating the effect of each drug separately and of their interaction (p-values given in the inset).

    Article Snippet: The used antibodies were against Chk1 phosphorylated at S345 (Cell Signaling Technology, #2348), Chk1 (Santa Cruz Biotechnology, #sc8408), Chk2 phosphorylated at T68 (Cell Signaling Technology, #2661S), Chk2 (Milipore, #05-649), TopBP1 (Euromedex, #A300-111A or Santa Cruz Biotechnology, #sc-271043), ATM phosphorylated at S1981 (Rockland, #200-301-400), α-tubulin (Sigma, #T5168), RPA phosphorylated at S33 (Abcam, #ab2118877), RPA (Abcam, #AB2175), GSK-3β phosphorylated at S9 (Cell Signaling Technology, #9336), GSK-3β (Cell Signaling Technology, #9832), PARP1 (Santa Cruz Biotechnology, #sc-8007), cleaved caspase-3 (Cell Signaling Technology, #9661).

    Techniques: Incubation, Immunofluorescence, Expressing, Imaging, Cytometry